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人包蟲抗體IgG ELISA檢測試劑盒
產品時間:2016-09-29
人包蟲抗體IgG ELISA檢測試劑盒,行業*,國內外多家ELISA試劑盒代理供應,價格相對平價,公司提供免費代測及ELISA試劑盒包郵服務。

本產品僅用于科研,不用于臨床

人包蟲抗體IgG ELISA檢測試劑盒,英文名:人包蟲抗體IgG ELISA試劑盒,英文縮寫:人包蟲抗體IgG ELISA試劑盒,常見試劑盒規格:96T/48T,產品別名:人包蟲抗體IgG ELISA試劑盒人包蟲抗體IgGELISA試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。人包蟲抗體IgG ELISA檢測試劑盒  在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質。可用洗板機洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。

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載脂蛋白A2(APOA2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Apolipoprotein A2 (APOA2)48T/96TY09763Rhesus monkey (Simian)
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多巴胺受體D2(DRD2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Dopamine Receptor D2 (DRD2)48T/96TY09765Rattus norvegicus (Rat)
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腫瘤蛋白p53(TP53)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tumor Protein p53 (TP53)48T/96TY09768Oryctolagus cuniculus (Rabbit)
Toll樣受體4(TLR4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Toll Like Receptor 4 (TLR4)48T/96TY09769Rattus norvegicus (Rat)
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 Y09783Homo sapiens (Human)
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各種類型ELISA測定時, 一般需經過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。

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    請輸入計算結果(填寫阿拉伯數字),如:三加四=7
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